Microbiologic and Histologic Assessment of Intentional Bacterial Contamination of Bone Grafts

Yaman, Ferhan; Ünlü, Gülten; Atilgan, Serhat; Celik, Yusuf; Özekinci, Tuncer; Yaldiz, Mehmet

doi:10.1016/j.joms.2006.10.027

« Previous Next »Journal of Oral and Maxillofacial Surgery
Volume 65, Issue 8 , Pages 1490-1494, August 2007

Microbiologic and Histologic Assessment of Intentional Bacterial Contamination of Bone Grafts

Ferhan Yaman, DDS, PhD
- Research Assistant, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
- Address correspondence and reprint requests to Dr Yaman: Department of Oral and Maxillofacial Surgery, School of Dentistry, Dicle University, 21280 Diyarbakir, Turkey
Gülten Ünlü, DDS, PhD
- Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
Serhat Atilgan, DDS, PhD
- Research Assistant, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
Yusuf Celik, PhD
- Professor, Department of Biostatistics, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.
Tuncer Özekinci, MD
- Associate Professor, Department of Microbiology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.
Mehmet Yaldiz, MD
- Associate Professor, Department of Pathology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.

Purpose

The purpose of this study was to investigate methods of removing pathogenic micro-organisms from bone grafts that have been contaminated during surgery.

Materials and Methods

Femora were removed from Sprague-Dawley rats and were divided into sections and contaminated in solutions of the bacteria Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Contaminated bone specimens in each group were immersed in various solutions for specified periods so their antibacterial effects could be evaluated. After these procedures were performed, bone specimens were cultured in nutrient media. Bone structure was evaluated, and the appropriate decontamination method was selected.

Results

Solutions such as povidone-iodine, neomycin, cephazolin sodium, and rifamycin were found to be effective decontaminants. These solutions did not damage the bone structure. Among these solutions, only rifamycin was effective against all bacteria used in this study to contaminate bone grafts.