Journal of Oral and Maxillofacial Surgery
Volume 65, Issue 8 , Pages 1490-1494 , August 2007

Microbiologic and Histologic Assessment of Intentional Bacterial Contamination of Bone Grafts

  • Ferhan Yaman, DDS, PhD

      Affiliations

    • Research Assistant, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
    • Corresponding Author InformationAddress correspondence and reprint requests to Dr Yaman: Department of Oral and Maxillofacial Surgery, School of Dentistry, Dicle University, 21280 Diyarbakir, Turkey
    • ,
  • Gülten Ünlü, DDS, PhD

      Affiliations

    • Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
    • ,
  • Serhat Atilgan, DDS, PhD

      Affiliations

    • Research Assistant, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
    • ,
  • Yusuf Celik, PhD

      Affiliations

    • Professor, Department of Biostatistics, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.
    • ,
  • Tuncer Özekinci, MD

      Affiliations

    • Associate Professor, Department of Microbiology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.
    • ,
  • Mehmet Yaldiz, MD

      Affiliations

    • Associate Professor, Department of Pathology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.

References 

  1. Hooe W, Steinberg B. Management of contaminated bone grafts: An experimental in vitro study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996;82:34
  2. Goebel ME, Drez D, Heck SB, Stoma MK. Contaminated rabbit patellar tendon grafts: In vivo analysis of disinfecting methods. Am J Sports Med. 1994;22:387
  3. Ayliffe GA, Collins BJ, Lowbury EJL. Cleaning and disinfection of hospital floors. Br J Med. 1966;2:442
  4. Humphreys H, Marshall RJ, Ricketts VE, Russell AJ, Reeves DS. Theatre over-shoes do not reduce operating theatre floor bacterial counts. J Hospital Infect. 1991;17:117
  5. Ellis E. Biology of bone grafting: An overview. In:  O’Ryan F editors. Selected Readings in Oral and Maxillofacial Surgery. vol 2:San Francisco, CA: The Guild for Scientific Advancement in Oral Maxillofacial Surgery; 1991;p. 1–28
  6. Dahners LE, Hoyle M. Chemical sterilization of bacterially contaminated bone without destruction of osteogenic potential. J Orthop Trauma. 1989;3:241
  7. Egyedi P, van Palenstein Helderman W. Sterilization of infected bone by lyophilization and rehydration with antibiotic solutions. J Maxillofac Surg. 1976;4:65
  8. Cruz NI, Cestero HJ, Cora ML. Management of contaminated bone grafts. Plast Reconstr Surg. 1981;68:411
  9. Rodeheaver G, Bellamy W, Kody M, et al. Bactericidal activity and toxicity of iodine-containing solutions in wounds. Arch Surg. 1982;117:181
  10. Christenson C, Jones R. Intra-operative contamination of bone and cartilage during an Austin bunionectomy. J Foot Surg. 1992;31:285
  11. Presnal BP, Kimbrough EE. What to do about a dropped bone graft. Clin Orthop. 1993;296:310
  12. Cooper DE, Arnoczky SP, Warren RF. Contaminated patellar tendon grafts: Incidence of positive cultures and efficacy of an antibiotic solution soak—An in vitro study. Arthroscopy. 1991;7:272
  13. Molina ME, Nonweiller DE, Evans JA, et al. Contaminated anterior cruciate ligament grafts: The efficacy of 3 sterilization agents. Arthroscopy. 2000;16:373
  14. Brown KL, Cruess RL. Bone and cartilage transplantation in orthopaedic surgery: A review. J Bone Joint Surg Am. 1982;64:270
  15. Friedlaender GE. Bone grafts: The basic science rationale for clinical applications. J Bone Joint Surg Am. 1987;69:786
  16. Tomford WW, Thongphasuk J, Mankin HJ, et al. Frozen musculoskeletal allografts: A study of the clinical incidence and causes of infection associated with their use. J Bone Joint Surg Am. 1990;72:1137
  17. Severyns AM, Lejeune A, Rocoux G, et al. Non-toxic antiseptic irrigation with chlorhexidine in experimental revascularization in the rat. J Hosp Infect. 1991;17:197
  18. Branemark PI, Ekholm R. Tissue injury caused by wound disinfectants. J Bone Joint Surg Am. 1967;49:48
  19. Soyer J, Rouil M, Castel O. The effect of 10% povidone-iodine solution on contaminated bone allografts. J Hosp Infect. 2002;50:183
  20. Stanford R, Solomon M, Levick M, et al. Decontamination of contaminated bone–tendon autografts using 10% povidone-iodine solution. Orthopedics. 1999;22:601
  21. Deijkers RL, Bloem RM, Petit PL, et al. Contamination of bone allografts: Analysis of incidence and predisposing factors. J Bone Joint Surg Br. 1997;79:161
  22. van Winkle BA, Neustein J. Management of open fractures with decontamination of large, contaminated, extruded cortical fragments. Clin Orthop. 1987;223:275

PII: S0278-2391(06)01944-6

doi: 10.1016/j.joms.2006.10.027

Journal of Oral and Maxillofacial Surgery
Volume 65, Issue 8 , Pages 1490-1494 , August 2007

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