Morphological and Immunohistochemical Changes in Muscle Tissue in Association With Mandibular Distraction Osteogenesis
Purpose
Studies of changes in the surrounding soft tissue in association with distraction osteogenesis in the maxillofacial region, where various different kinds of tissue are tightly packed, are rare. In this study, we performed morphological and immunohistochemical investigations of muscle tissue during mandibular distraction osteogenesis.
Materials and Methods
Japanese white rabbits were divided into 2 groups. In 1 group, the mandibular bone was distracted at a rate of 1 mm/day (DO group), whereas in the other group it was advanced by 10 mm all at once (O group). The cross-section of the anterior belly of the digastric muscle was examined using hematoxylin and eosin, periodic acid-Schiff (PAS), and proliferating cell nuclear antigen (PCNA) staining methods, as well as scanning electronmicroscopy (SEM).
Results
In the DO group, the cross-section of muscle bundle revealed no remarkable changes. In PAS staining, type II fibers gradually disappeared with distraction, but eventually reappeared after a period of consolidation. In the SEM photographs, striation-like laminated structures were evident in the control and the 3-mm distraction, ambiguous in the 6-mm distraction, and not evident in the 10-mm distraction. In contrast, the structure appeared to be restored in the consolidation for 1 week thereafter. In the PCNA immunostaining, the numbers of PCNA-positive nuclei increased during periods of distraction, but subsequently tended to decrease gradually.
Conclusions
Although minute injuries were induced in muscle fibers in association with distraction osteogenesis, it is suggested that muscle fibers regenerate starting in the distraction period and thus can adapt to the environment.
⁎Graduate Student, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
†Senior Assistant Professor, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
‡Graduate Student, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
§Assistant Professor, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
∥Assistant Professor, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
¶Instrumental Analysis Research Center, Tokyo Medical and Dental University, Tokyo, Japan.
#Dentistry and Oral Surgery, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan.
⁎⁎Professor, Oral and Maxillofacial Surgery, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Address correspondence and reprint requests to Dr Sato: Tokyo Medical and Dental University, Division of Oral Health Sciences, Graduate School, Department of Oral Restitution, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
ABSTRACT