Morphological and Immunohistochemical Changes in Muscle Tissue in Association With Mandibular Distraction Osteogenesis

Sato, Masaru; Maruoka, Yutaka; Kunimori, Kentaro; Imai, Hideki; Kabasawa, Yuji; Ichinose, Shizuko; Harada, Kiyoshi; Omura, Ken

doi:10.1016/j.joms.2006.10.041

« Previous Next »Journal of Oral and Maxillofacial Surgery
Volume 65, Issue 8 , Pages 1517-1525, August 2007

Morphological and Immunohistochemical Changes in Muscle Tissue in Association With Mandibular Distraction Osteogenesis

Masaru Sato, DDS
- Graduate Student, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
- Address correspondence and reprint requests to Dr Sato: Tokyo Medical and Dental University, Division of Oral Health Sciences, Graduate School, Department of Oral Restitution, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
Yutaka Maruoka, DDS, PhD
- Senior Assistant Professor, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Kentaro Kunimori, DDS
- Graduate Student, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Hideki Imai, DDS, PhD
- Assistant Professor, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Yuji Kabasawa, DDS, PhD
- Assistant Professor, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Shizuko Ichinose, PhD
- Instrumental Analysis Research Center, Tokyo Medical and Dental University, Tokyo, Japan.
Kiyoshi Harada, DDS, PhD
- Dentistry and Oral Surgery, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan.
Ken Omura, DDS, PhD
- Professor, Oral and Maxillofacial Surgery, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.

Purpose

Studies of changes in the surrounding soft tissue in association with distraction osteogenesis in the maxillofacial region, where various different kinds of tissue are tightly packed, are rare. In this study, we performed morphological and immunohistochemical investigations of muscle tissue during mandibular distraction osteogenesis.

Materials and Methods

Japanese white rabbits were divided into 2 groups. In 1 group, the mandibular bone was distracted at a rate of 1 mm/day (DO group), whereas in the other group it was advanced by 10 mm all at once (O group). The cross-section of the anterior belly of the digastric muscle was examined using hematoxylin and eosin, periodic acid-Schiff (PAS), and proliferating cell nuclear antigen (PCNA) staining methods, as well as scanning electronmicroscopy (SEM).

Results

In the DO group, the cross-section of muscle bundle revealed no remarkable changes. In PAS staining, type II fibers gradually disappeared with distraction, but eventually reappeared after a period of consolidation. In the SEM photographs, striation-like laminated structures were evident in the control and the 3-mm distraction, ambiguous in the 6-mm distraction, and not evident in the 10-mm distraction. In contrast, the structure appeared to be restored in the consolidation for 1 week thereafter. In the PCNA immunostaining, the numbers of PCNA-positive nuclei increased during periods of distraction, but subsequently tended to decrease gradually.

Conclusions

Although minute injuries were induced in muscle fibers in association with distraction osteogenesis, it is suggested that muscle fibers regenerate starting in the distraction period and thus can adapt to the environment.