Journal of Oral and Maxillofacial Surgery
Volume 66, Issue 2 , Pages 297-301 , February 2008

Effects of Activated and Nonactivated Platelet-Rich Plasma on Proliferation of Human Osteoblasts In Vitro

  • Jan Slapnicka, MD, DDS

      Affiliations

    • Oral and Maxillofacial Surgery Resident, Department of Oral and Maxillofacial Surgery, University Hospital Brno Bohunice; and Dental Research Center, Masaryk University, Brno, Czech Republic.
    • Corresponding Author InformationAddress correspondence and reprint requests to Dr Slapnicka: Department of Oral and Maxillofacial Surgery, University Hospital Brno Bohunice, Jihlavska 20, Brno 62500, Czech Republic
    • ,
  • Antonin Fassmann, DDS, PhD

      Affiliations

    • Chief of Periodontology, Clinic of Stomatology, St Anne’s University Hospital, Medical Faculty; and Dental Research Center, Masaryk University; Brno, Czech Republic.
    • ,
  • Ludek Strasak, PhD

      Affiliations

    • Research Fellow, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
    • ,
  • Peter Augustin, DDS

      Affiliations

    • Periodontology Resident, Clinic of Stomatology, St Anne’s University Hospital, Medical Faculty; and Dental Research Center, Masaryk University, Brno, Czech Republic.
    • ,
  • Jiri Vanek, DDS, PhD

      Affiliations

    • Director of Dental Research Center, Clinic of Stomatology, St Anne’s University Hospital, Medical Faculty; and Dental Research Center, Masaryk University; Brno, Czech Republic.

References 

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  2. Anitua E, Andia I, Ardanza B, et al. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004;91:4
  3. Mendez R, Lopez-Cedrun JL, Patino B, et al. Platelet-rich plasma (platelet gel) in secondary alveoloplasty in cleft patients. Cir Pediatr. 2006;19:23
  4. Marx RE. Platelet concentrate: A strategy for accelerating and improving bone regeneration. In:  Davies JE editors. Bone Engineering. Toronto: University of Toronto; 2000;p. 447–453
  5. Okuda K, Tai H, Tanabe K, et al. Platelet-rich plasma combined with a porous hydroxyapatite graft for the treatment of intrabony periodontal defects in humans: A comparative controlled clinical study. J Periodontol. 2005;76:890
  6. Sammartino G, Tia M, Marenzi G, et al. Use of autologous platelet-rich plasma (PRP) in periodontal defect treatment after extraction of impacted mandibular third molars. J Oral Maxillofac Surg. 2005;63:766
  7. Bhanot S, Alex JC. Current applications of platelet gels in facial plastic surgery. Facial Plast Surg. 2002;18:27
  8. Man D, Plosker H, Winland-Brown J. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg. 2001;107:238
  9. Welsh WJ. Autologous platelet gel-clinical function and usage in plastic surgery. Cosmetic Derm. 2000;13:13
  10. Gandhi A, Bibbo C, Pinzur M, et al. The role of platelet-rich plasma in foot and ankle surgery. Foot Ankle Clin. 2005;10:621;viii
  11. Kinghton DR, Ciresi K, Fiegel VD, et al. Stimulation of repair in chronic, nonhealing, cutaneous ulcers using platelet-derived wound healing formula. Surg Gynecol Obstet. 1990;170:56
  12. Klongnoi B, Rupprecht S, Kessler P, et al. Influence of platelet-rich plasma on a bioglass and autogenous bone in sinus augmentation. Clin Oral Implants Res. 2006;17:312
  13. Gerard D, Carlson ER, Gotcher JE, et al. Effects of platelet-rich plasma on the healing of autologous bone grafted mandibular defects in dogs. J Oral Maxillofac Surg. 2006;64:443
  14. Furst G, Gruber R, Tangl S, et al. Sinus grafting with autogenous platelet-rich plasma and bovine hydroxyapatite (A histomorphometric study in minipigs). Clin Oral Implants Res. 2003;14:500
  15. Sanchez AR, Sheridan PJ, Eckert SE, et al. Regenerative potential of platelet-rich plasma added to xenogenic bone grafts in peri-implant defects: A histomorphometric analysis in dogs. J Periodontol. 2005;76:1637
  16. Thorwarth M, Wehrhan F, Schultze-Mosgau S, et al. PRP modulates expression of bone matrix proteins in vivo without long-term effects on bone formation. Bone. 2006;38:30
  17. Dallari D, Fini M, Stagni C, et al. In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet-rich plasma and freeze-dried bone allografts, alone and in combination. J Orthop Res. 2006;24:877
  18. Pryor ME, Polimeni G, Koo KT, et al. Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: Histologic and histometric observations. J Clin Periodontol. 2005;32:966
  19. Graziani F, Ivanovski S, Cei S, Ducci F, et al. The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clin Oral Implants Res. 2006;17:212
  20. Ferreira CF, Carriel Gomes MC, Filho JS, et al. Platelet-rich plasma influence on human osteoblasts growth. Clin Oral Implants Res. 2005;16:456
  21. Kanno T, Takahashi T, Tsujisawa T, et al. Platelet-rich plasma enhances human osteoblast-like cell proliferation and differentiation. J Oral Maxillofac Surg. 2005;63:362
  22. Cenni E, Ciapetti G, Pagani S, et al. Effects of activated platelet concentrates on human primary cultures of fibroblasts and osteoblasts. J Periodontol. 2005;76:323
  23. Vick VL, Holds JB, Hartstein ME, et al. Use of autologous platelet concentrate in blepharoplasty surgery. Ophthal Plast Reconstr Surg. 2006;22:102
  24. Huang LH, Neiva RE, Soehren SE, et al. The effect of platelet-rich plasma on the coronally advanced flap root coverage procedure: A pilot human trial. J Periodontol. 2005;76:1768
  25. Graziani F, Ivanovski S, Cei S, et al. The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clin Oral Implants Res. 2006;17:212

 This research project was supported by Dental Research Center (project no 1M0021622409), Masaryk University, Brno, Czech Republic.

PII: S0278-2391(07)00642-8

doi: 10.1016/j.joms.2007.05.022

Journal of Oral and Maxillofacial Surgery
Volume 66, Issue 2 , Pages 297-301 , February 2008

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