Histomorphometric Evaluation of the Effects of Zoledronic Acid on Mandibular Distraction Osteogenesis in Rabbits
Purpose
To assess the effect of systemic administration of zoledronic acid (ZA) on mineralization of newly formed bone and to determine strain-related osteoporosis on surrounding bone during lengthening of immature rabbit mandible.
Materials and Methods
Eighteen New Zealand white rabbits were divided randomly into 2 groups, and bone lengthening was carried out in the left portion of the mandible through distraction osteogenesis with a rate of 0.5 mm every 12 hours for 5 days. The experimental group was administered 0.1 mg/kg ZA intravenously. The control group was given saline infusion only during operation. All animals were sacrificed at the end of the 28-day consolidation period. The mandibles of all animals were removed and regenerate was evaluated. Osteoblasts, osteoclasts, collagen fibers, and fibroblasts were marked within 0.1-mm2 area and newly formed bone area was measured within 0.5-mm2 area. All data were analyzed using Mann-Whitney U test.
Results
Although irregular bone destruction spots were seen in the control group, the experimental group showed regular ossification areas and significant difference between osteoblast and osteoclast numbers (P < .05). In the regenerate zone, there was considerable difference between the 2 groups in terms of osteoblast, osteoclast, and collagen amounts (P < .05). Additionally, newly formed bone areas and fibroblast count were higher in experimental group.
Conclusions
The results of this study showed that ZA had positive effects on the new bone formation, which may potentially shorten the consolidation period.
⁎Assistant Professor, Department of Oral and Maxillofacial Surgery, Karadeniz Technical University, Faculty of Dentistry, Trabzon, Turkey.
†Associate Professor, Department of Oral and Maxillofacial Surgery, Selcuk University Faculty of Dentistry, Konya, Turkey.
‡Associate Professor, Department of Oral and Maxillofacial Surgery, Kırıkkale University, Faculty of Dentistry, Kırıkkale, Turkey.
§Professor, Department of Pathology, Meram Medical Faculty, Selcuk University, Konya, Turkey.
¶Professor, Department of Oral and Maxillofacial Surgery, Ankara University Faculty of Dentistry, Ankara, Turkey.
Address correspondence and reprint requests to Dr Pampu: Selcuk University Faculty of Dentistry, Oral and Maxillofacial Surgery, Selcuk Universitesi Dis Hekimligi, Agiz Dis Cene Hastaliklari ve Cer, 42079 Konya, Turkey
This study was supported by Scientific Research Project, Selçuk University, Konya, Turkey.
ABSTRACT