Journal of Oral and Maxillofacial Surgery
Volume 67, Issue 2 , Pages 265-272 , February 2009

Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Healing of Standardized Bone Defects in the Alveolar Ridge: A Comparative Histomorphometric Study in Minipigs

  • Francesco Pieri, DDS, PhD

      Affiliations

    • Research Fellow, Department of Odontostomatological Sciences, University of Bologna, Bologna, Italy
    • Corresponding Author InformationAddress correspondence and reprint requests to Dr Pieri: Via San Vitale 59, 40136 Bologna, Italy
    • ,
  • Enrico Lucarelli, BS

      Affiliations

    • Research Associate, Bone Regeneration Laboratory, Istituti Ortopedici Rizzoli, Bologna, Italy
    • ,
  • Giuseppe Corinaldesi, MD, DDS

      Affiliations

    • Research Associate, Department of Odontostomatological Sciences, University of Bologna, Bologna, Italy
    • ,
  • Milena Fini, MD

      Affiliations

    • Research Associate, Experimental Surgery Laboratory, Rizzoli Orthopaedic Institute, Bologna, Italy
    • ,
  • Nicolò Nicoli Aldini, MD

      Affiliations

    • Research Associate, Experimental Surgery Laboratory, Rizzoli Orthopaedic Institute, Bologna, Italy
    • ,
  • Roberto Giardino, MD

      Affiliations

    • Professor and Director, Experimental Surgery Laboratory, Rizzoli Orthopaedic Institute, Bologna, Italy
    • ,
  • Davide Donati, MD

      Affiliations

    • Professor and Director, Bone Regeneration Laboratory, Istituti Ortopedici Rizzoli, Bologna, Italy
    • ,
  • Claudio Marchetti, MD, DDS

      Affiliations

    • Professor and Director, Department of Odontostomatological Sciences, University of Bologna, Bologna, Italy

References 

  1. Araújo MG, Lindhe J. Dimensional ridge alterations following tooth extraction (An experimental study in the dog). J Clin Periodontol. 2005;32:212
  2. von Arx T, Cochran DL, Hermann JS, et al. Lateral ridge augmentation using different bone fillers and barrier membrane application (A histologic and histomorphometric pilot study in the canine mandible). Clin Oral Implants Res. 2001;12:260
  3. Joshi A, Kostakis GC. An investigation of post-operative morbidity following iliac crest graft harvesting. Br Dent J. 2004;196:167
  4. Iasella JM, Greenwell H, Miller RL, et al. Ridge preservation with freeze-dried bone allograft and a collagen membrane compared to extraction alone for implant site development: A clinical and histologic study in humans. J Periodontol. 2003;74:990
  5. Indovina A, Block MS. Comparison of 3 bone substitutes in canine extraction sites. J Oral Maxillofac Surg. 2002;60:53
  6. Thompson DM, Rohrer MD, Prasad HS. Comparison of bone grafting materials in human extraction sockets: Clinical, histologic, and histomorphometric evaluations. Implant Dent. 2006;15:89
  7. Krebsbach PH, Kuznetsov SA, Bianco P, et al. Bone marrow stromal cells: Characterization and clinical application. Crit Rev Oral Biol Med. 1999;10:165
  8. Shanti RM, Nesti LJ, Wang X, et al. Adult mesenchymal stem cells: Biological properties, characteristics, and applications in maxillofacial surgery. J Oral Maxillofac Surg. 2007;65:1640
  9. Chen F, Chen S, Ding G. Bone graft in the shape of human mandibular condyle reconstruction via seeding marrow-derived osteoblasts into porous coral in a nude mice model. J Oral Maxillofac Surg. 2002;60:1155
  10. Ueda M, Yamada Y, Ozawa R, et al. Clinical case reports of injectable tissue-engineered bone for alveolar augmentation with simultaneous implant placement. Int J Periodontics Restorative Dent. 2005;25:129
  11. Hasegawa N, Kawaguchi H, Hirachi A, et al. Behavior of transplanted bone marrow-derived mesenchymal stem cells in periodontal defects. J Periodontol. 2006;77:1003
  12. Marx R, Carlson E, Eichstaedt R, et al. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85:638
  13. Aghaloo TL, Moy PK, Freymiller EG. Investigation of platelet-rich plasma in rabbit cranial defects (An animal study). J Oral Maxillofac Surg. 2002;60:1176
  14. Lucarelli E, Beccheroni A, Donati E, et al. Platelet-derived growth factors enhance proliferation of human stromal stem cells. Biomaterials. 2003;24:3095
  15. Kandler B, Fugl A, Gabriele M, et al. Bone marrow stromal cells of young and adult rats respond similarly to platelet-released supernatant and bone morphogenetic protein-6 in vitro. J Periodontol. 2006;77:699
  16. Yamada Y, Boo JS, Ozawa R, et al. Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold. J Craniomaxillofac Surg. 2003;31:27
  17. Yamada Y, Ueda M, Naiki T, et al. Tissue-engineered injectable bone regeneration for osseointegrated dental implants. Clin Oral Implants Res. 2004;15:589
  18. Hollister SJ, Lin CY, Saito E, et al. Engineering craniofacial scaffolds. Orthod Craniofac Res. 2005;8:162
  19. Turhani D, Watzinger E, Weissenböck M, et al. Analysis of cell-seeded 3-dimensional bone constructs manufactured in vitro with hydroxyapatite granules obtained from red algae. J Oral Maxillofac Surg. 2005;63:673
  20. Turhani D, Cvikl B, Watzinger E, et al. In vitro growth and differentiation of osteoblast-like cells on hydroxyapatite ceramic granule calcified from red algae. J Oral Maxillofac Surg. 2005;63:793
  21. Weibrich G, Hansen T, Kleis W, et al. Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration. Bone. 2004;34:665
  22. Lucarelli E, Fini M, Beccheroni A, et al. Stromal stem cells and platelet-rich plasma improve bone allograft integration. Clin Orthop Relat Res. 2005;435:62
  23. Cardaropoli G, Araújo M, Hayacibara R, et al. Healing of extraction sockets and surgically produced—augmented and non-augmented—defects in the alveolar ridge (An experimental study in the dog). J Clin Periodontol. 2005;32:435
  24. De Kok IJ, Drapeau SJ, Young R, et al. Evaluation of mesenchymal stem cells following implantation in alveolar sockets: A canine safety study. Int J Oral Maxillofac Implants. 2005;20:511
  25. Buser D, Hoffmann B, Bernard JP, et al. Evaluation of filling materials in membrane-protected bone defects (A comparative histomorphometric study in the mandible of miniature pigs). Clin Oral Implants Res. 1998;9:137
  26. Carmagnola D, Berglundh T, Lindhe J. The effect of a fibrin glue on the integration of Bio-Oss with bone tissue (An experimental study in Labrador dogs). J Clin Periodontol. 2002;29:377
  27. Yamada Y, Nagasaka T, Baba S, et al. Osteogenic potential of injectable tissue-engineered bone: A comparison among autogenous bone, bone substitute (Bio-oss), platelet-rich plasma, and tissue-engineered bone with respect to their mechanical properties and histological findings. J Biomed Mater Res A. 2005;73:63
  28. Hou R, Chen F, Yang Y, et al. Comparative study between coral-mesenchymal stem cells-rhBMP-2 composite and auto-bone-graft in rabbit critical-sized cranial defect model. J Biomed Mater Res A. 2007;80:85
  29. Saka B, Wree A, Anders L, et al. Experimental and comparative study of the blood supply to the mandibular cortex in Gottingen minipigs and in man. J Craniomaxillofac Surg. 2002;30:219
  30. Jensen SS, Broggini N, Weibrich G, et al. Bone regeneration in standardized bone defects with autografts or bone substitutes in combination with platelet concentrate: A histologic and histomorphometric study in the mandibles of minipigs. Int J Oral Maxillofac Implants. 2005;20:703
  31. Fuerst G, Gruber R, Tangl S, et al. Effect of platelet released growth factors and collagen type I on osseous regeneration of mandibular defects (A pilot study in minipigs). J Clin Periodontol. 2004;31:784
  32. Henkel KO, Gerber T, Dörfling P, et al. Repair of bone defects by applying biomatrices with and without autologous osteoblasts. J Craniomaxillofac Surg. 2005;33:45
  33. Ewers R, Goriwoda W, Schopper C, et al. Histologic findings at augmented bone areas supplied with two different bone substitute materials combined with sinus floor lifting (Report of one case). Clin Oral Implants Res. 2004;15:96
  34. Simunek A, Cierny M, Kopecka E, et al. The sinus lift with phycogenic bone substitute (A histomorphometric study). Clin Oral Implants Res. 2005;16:342
  35. Schliephake H, Knebel JW, Aufderheide M, et al. Use of cultivated osteoprogenitor cells to increase bone formation in segmental mandibular defects: An experimental pilot study in sheep. Int J Oral Maxillofac Surg. 2001;30:531
  36. Abukawa H, Shin M, Williams WB, et al. Reconstruction of mandibular defects with autologous tissue engineered bone. J Oral Maxillofac Surg. 2004;62:601
  37. Yuan J, Cui L, Zhang WJ, et al. Repair of canine mandibular bone defects with bone marrow stromal cells and porous beta-tricalcium phosphate. Biomaterials. 2007;28:1005
  38. Mankani MH, Kuznetsov SA, Wolfe RM, et al. In vivo bone formation by human bone marrow stromal cells: Reconstruction of the mouse calvarium and mandible. Stem Cells. 2006;24:2140
  39. Ogino Y, Ayukawa Y, Kukita T, et al. The contribution of platelet-derived growth factor, transforming growth factor-beta1, and insulin-like growth factor-I in platelet-rich plasma to the proliferation of osteoblast-like cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:724
  40. Fennis JP, Stoelinga PJ, Jansen JA. Mandibular reconstruction: a histological and histomorphometric study on the use of autogenous scaffolds, particulate corticocancellous bone grafts and platelet rich plasma in goats. Int J Oral Maxillofac Surg. 2004;33:48
  41. Gerard D, Carlson ER, Gotcher JE, et al. Effects of platelet-rich plasma at the cellular level on healing of autologous bone-grafted mandibular defects in dogs. J Oral Maxillofac Surg. 2007;65:721
  42. Vogel JP, Szalay K, Geiger F, et al. Platelet-rich plasma improves expansion of human mesenchymal stem cells and retains differentiation capacity and in vivo bone formation in calcium phosphate ceramics. Platelets. 2006;17:462
  43. Wiltfang J, Kloss FR, Nkenke E, et al. Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects (An animal experiment). Clin Oral Implant Res. 2004;15:187
  44. Jung RE, Schmoekel HG, Zwahlen R, et al. Platelet-rich plasma and fibrin as delivery systems for rhBMP-2. Clin Oral Impl Res. 2005;16:676

PII: S0278-2391(08)01075-6

doi: 10.1016/j.joms.2008.06.036

Journal of Oral and Maxillofacial Surgery
Volume 67, Issue 2 , Pages 265-272 , February 2009

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