Journal of Oral and Maxillofacial Surgery
Volume 67, Issue 2 , Pages 257-264 , February 2009

Novel Approach to Engineer Implantable Nasal Alar Cartilage Employing Marrow Precursor Cell Sheet and Biodegradable Scaffold

  • Junrui Zhang, DDS

      Affiliations

    • Associate Professor, Department of Oral and Maxillofacial Surgery, Qindu Hospital, the Fourth Military Medical University, Xi'an, People's Republic of China
    • ,
  • Liangqi Liu, MS

      Affiliations

    • Student, Rege Lab of Tissue Engineering, Faculty of Life Science Northwest University, Xi'an, P.R. China
    • ,
  • Zhan Gao, DDS

      Affiliations

    • Associate Professor, Department of Oral and Maxillofacial Surgery, Qindu Hospital, the Fourth Military Medical University, Xi'an, P.R. China
    • ,
  • Liwen Li, PhD

      Affiliations

    • Associate Professor, Rege Lab of Tissue Engineering, Faculty of Life Science Northwest University, Xi'an, P.R. China
    • ,
  • Xinghua Feng, DDS

      Affiliations

    • Professor, Department of Oral and Maxillofacial Surgery, Qindu Hospital, the Fourth Military Medical University, Xi'an, P.R. China
    • ,
  • Wei Wu, DDS

      Affiliations

    • Resident, Department of Oral and Maxillofacial Surgery, Qindu Hospital, the Fourth Military Medical University, Xi'an, P.R. China
    • ,
  • Qin Ma, DDS

      Affiliations

    • Associate Professor, Department of Oral and Maxillofacial Surgery, Qindu Hospital, the Fourth Military Medical University, Xi'an, P.R. China
    • ,
  • Xiaobing Cheng, DDS

      Affiliations

    • Associate Professor, Department of Oral and Maxillofacial Surgery, Qindu Hospital, the Fourth Military Medical University, Xi'an, P.R. China
    • ,
  • Fulin Chen, PhD

      Affiliations

    • Professor, Rege Lab of Tissue Engineering, Faculty of Life Science Northwest University, Xi'an, P.R. China
    • ,
  • Tianqiu Mao, DDS

      Affiliations

    • Professor, Department of Oral and Maxillofacial Surgery, Qindu Hospital, the Fourth Military Medical University, Xi'an, P.R. China
    • Corresponding Author InformationAddress correspondence and reprint requests to Dr Mao: Department of Oral and Maxillofacial Surgery, Qindu Hospital, the Fourth Military Medical University, Chang Le Xi Road 145#, Xi'an, P.R. China 710032

References 

  1. Wu W, Chen F, Liu Y, et al. Autologous injectable tissue-engineered cartilage by using platelet-rich plasma: Experimental study in a rabbit model. J Oral Maxillofac Surg. 2007;65:1951
  2. Ruszymah BH, Chua K, Latif MA, et al. Formation of in vivo tissue engineered human hyaline cartilage in the shape of a trachea with internal support. Int J Pediatr Otorhinolaryngol. 2005;69:1489
  3. Cao Y, Vacanti JP, Paige KT, et al. Transplantation of chondrocytes utilizing a polymer-cell construct to produce tissue-engineered cartilage in the shape of a human ear. Plast Reconstr Surg. 1997;100:297
  4. Kojima K, Bonassar LJ, Ignotz RA, et al. Comparison of tracheal and nasal chondrocytes for tissue engineering of the trachea. Ann Thorac Surg. 2003;76:1884
  5. Honda MJ, Yada T, Ueda M, et al. Cartilage formation by serial passaged cultured chondrocytes in a new scaffold: hybrid 75:25 poly(L-lactide-epsilon-c-aprolactone) sponge. J Oral Maxillofac Surg. 2004;62:1510
  6. Thirion S, Berenbaum F. Culture and phenotyping of chondrocytes in primary culture. Methods Mol Med. 2004;100:1
  7. Shanti RM, Li W-J, Nesti LJ, et al. Adult mesenchymal stem cells: Biological properties, characteristics, and applications in maxillofacial surgery. J Oral Maxillofac Surg. 2007;65:1640
  8. Yanada S, Ochi M, Kojima K, et al. Possibility of selection of chondrogenic progenitor cells by telomere length in FGF-2-expanded mesenchymal stromal cells. Cell Prolif. 2006;39:575
  9. Sekiya I, Vuoristo JT, Larson BL, et al. In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis. Proc Natl Acad Sci USA. 2002;99:4397
  10. Frenkel SR, Di Cesare PE. Scaffolds for articular cartilage repair. Ann Biomed Eng. 2004;32:26
  11. Mercier NR, Costantino HR, Tracy MA, et al. Poly(lactide-co-glycolide) microspheres as a moldable scaffold for cartilage tissue engineering. Biomaterials. 2005;26:1945
  12. Alpar B, Leyhausen G, Gunay H, et al. Compatibility of resorbable and nonresorbable guided tissue regeneration membranes in cultures of primary human periodontal ligament fibroblasts and human osteoblast-like cells. Clin Oral Invest. 2000;4:219
  13. Hammerle CH, Jung RE. Bone augmentation by means of barrier membranes. Periodontolgy. 2003;33:36
  14. Farndale RW, Buttle DJ, Barrett AJ. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta. 1986;883:173
  15. Sittinger M, Hutmacher DW, Risbud MV. Current strategies for cell delivery in cartilage and bone regeneration. Curr Opin Biotechnol. 2004;15:411
  16. Asplund B, Sperens J, Mathisen T, et al. Effects of hydrolysis on a new biodegradable co-polymer. J Biomater Sci Polym Ed. 2006;17:615
  17. Grayson AC, Voskerician G, Lynn A, et al. Differential degradation rates in vivo and in vitro of biocompatible poly(lactic acid) and poly(glycolic acid) homo- and co-polymers for a polymeric drug-delivery microchip. J Biomater Sci Polym Ed. 2004;15:1281
  18. Park K, Huang J, Azar F, et al. Scaffold-free, engineered porcine cartilage construct for cartilage defect repair—In vitro and in vivo study. Artif Organs. 2006;30:586
  19. Yang J, Yamato M, Kohno C, et al. Cell sheet engineering: Recreating tissues without biodegradable scaffolds. Biomaterials. 2005;26:6415
  20. Wu W, Chen F, Feng X. Engineering cartilage tissues with the shape of human nasal alar by using chondrocyte macroaggregate. J Biotechnol. 2007;130:75
  21. Hofmann S, Knecht S, Langer R, et al. Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells. Tissue Eng. 2006;12:2729
  22. Vilquin JT, Rosset P. Mesenchymal stem cells in bone and cartilage repair: Current status. Regen Med. 2006;1:589

 This study was supported by the National Natural Science Foundation of P.R. China (30770542) and The Education Department of Shaanxi Province (07JK415).

PII: S0278-2391(08)01392-X

doi: 10.1016/j.joms.2008.08.009

Journal of Oral and Maxillofacial Surgery
Volume 67, Issue 2 , Pages 257-264 , February 2009

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