In Vitro Osteogenic Differentiation of Cultured Human Dental Papilla-Derived Cells
Purpose
This study examined the osteogenic phenotypes and mineralization of cultured human dental papilla-derived cells.
Materials and Methods
Dental papillae were harvested from mandibles during surgical extraction of lower impacted third molars from 3 patients aged 13 to 15 years. The dental papilla-derived cells were introduced into the cell culture. After passage 3, the dental papilla-derived cells were further cultured for 42 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. We examined the histochemical detection of alkaline phosphatase (ALP), the reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for ALP and osteocalcin, and von Kossa staining in the dental papilla-derived cells.
Results
It was observed that ALP was strongly expressed in the earlier stage of osteoblastic differentiation, whereas osteocalcin was mainly expressed and secreted into the medium at the later stage. Von Kossa-positive mineralization nodules were first observed on day 14, which increased in number during the entire culture period.
Conclusions
These results suggest that dental papilla-derived cell have osteogenic potential and could be used as an additional source of cells for bone tissue engineering.
⁎Assistant Professor, Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsangnam-do, South Korea
†Research Professor, Clinical Research Institute, Gyeongsang National University Hospital, Gyeongsangnam-do, South Korea
‡Clinical Professor, Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsangnam-do, South Korea
§Postgraduate, Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsangnam-do, South Korea
‖Postgraduate, Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Gyeongsangnam-do, South Korea
¶Professor, Department of Biochemistry, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsangnam-do, South Korea
#Professor, Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Ami-dong, Busan, South Korea
⁎⁎Assistant Professor, Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsangnam-do, South Korea
Address correspondence and reprint requests to Dr Byun: Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, 660-702, Chilam-dong 90, Jinju, Gyeongsangnam-do, South Korea
1 Bong-Wook Park and Young-Sool Hah contributed equally to this work.
ABSTRACT