Journal of Oral and Maxillofacial Surgery
Volume 68, Issue 2 , Pages 260-267, February 2010

Effect of Intermittent Systemic Administration of Recombinant Parathyroid Hormone (1-34) on Mandibular Fracture Healing in Rats

  • Henry H. Rowshan, DDS

      Affiliations

    • Formerly, Chief Resident, Department of Oral and Maxillofacial Surgery, Dwight David Eisenhower Army Medical Center, Fort Gordon, GA; and Currently, Chief, Oral and Maxillofacial Surgery, Ft Irwin, CA
    • ,
  • Mary A. Parham, DVM

      Affiliations

    • Chief of Veterinary Medicine, Department of Clinical Investigation, Dwight David Eisenhower Army Medical Center, Fort Gordon, GA
    • ,
  • Dale A. Baur, DDS, MD

      Affiliations

    • Associate Professor and Chair, Department of Oral and Maxillofacial Surgery, Case Western Reserve University, Cleveland, OH
    • Corresponding Author InformationAddress correspondence and reprint requests to Dr Baur: Department of Oral and Maxillofacial Surgery, Case Western Reserve University/University Hospitals, 2123 Abington Drive, Cleveland, OH 44106-4905
    • ,
  • RaeLynn D. McEntee, BBA

      Affiliations

    • Research Specialist, Department of Clinical Investigation, Dwight David Eisenhower Army Medical Center, Fort Gordon, GA
    • ,
  • Eugene Cauley

      Affiliations

    • Registered Animal Technician, Department of Clinical Investigation, Dwight David Eisenhower Army Medical Center, Fort Gordon, GA
    • ,
  • Dane T. Carriere

      Affiliations

    • Research Specialist, Department of Clinical Investigation, Dwight David Eisenhower Army Medical Center, Fort Gordon, GA
    • ,
  • Joseph C. Wood, PhD, MD

      Affiliations

    • Chief, Department of Clinical Investigation, Dwight David Eisenhower Army Medical Center, Fort Gordon, GA
    • ,
  • William J. Demsar, DMD, MS

      Affiliations

    • Chief of Oral and Maxillofacial Pathology, Department of Oral and Maxillofacial Surgery, Dwight David Eisenhower Army Medical Center, Fort Gordon, GA
    • ,
  • Jose M. Pizarro, MS, PhD

      Affiliations

    • Officer in Charge, Department of Clinical Investigation, Dwight David Eisenhower Army Medical Center, Fort Gordon, GA

Purpose

To establish a rat mandibular fracture model and investigate the short- and long-term effects of recombinant parathyroid hormone (PTH 1-34) on mandibular fracture healing in rats.

Materials and Methods

A controlled unilateral mandibular fracture was created surgically in 29 male Sprague-Dawley rats and then stabilized using an external fixation device. The rats were divided into 2 groups: 1 group received daily subcutaneous injections of 10 μg/kg of PTH(1-34) and 1 group served as the vehicle control. The rats were killed on postoperative days 7 and 21, and radiographic densitometry and histologic evaluation of new bone formation were performed.

Results

A novel unilateral mandibular fracture model was established that has significant differences from previously published models, both in the location of the osteotomy site and in the rigid external stabilization device. The PTH(1-34) treated rats showed a statistically significant difference (P < .05) in callous formation compared with the control animals. Radiographic densitometry evaluation of the injury site revealed an increase in bone density, apparent at day 7 in the experimental group. Visual inspection of the histologic sections stained with Masson's trichrome blue showed an apparent increase in new bone formation at 21 days in the PTH-treated group compared with the control group.

Conclusions

Intermittent systemic administration of PTH(1-34) might enhance the healing of mandibular fractures in the early phase (7-day period). Long-term administration (21-day period) showed no statistically significant differences between the control and experimental group by radiographic densitometry.

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 This research was supported by internal funding from Southeast Regional Dental Command and Dental Activity and Dwight David Eisenhower Army Medical Center. The views of the authors do not purport to reflect the position of the Department of the Army or Department of Defense (paragraph 4-3), AR 360-5.

PII: S0278-2391(09)01749-2

doi:10.1016/j.joms.2009.09.045

Journal of Oral and Maxillofacial Surgery
Volume 68, Issue 2 , Pages 260-267, February 2010

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