Cell Response of Titanium Implant With a Roughened Surface Containing Titanium Hydride: An In Vitro Study

Zhang, Feng; Yang, Gou-li; He, Fu-ming; Zhang, Li-juan; Zhao, Shi-fang

doi:10.1016/j.joms.2009.12.027

« Previous Next »Journal of Oral and Maxillofacial Surgery
Volume 68, Issue 5 , Pages 1131-1139, May 2010

Cell Response of Titanium Implant With a Roughened Surface Containing Titanium Hydride: An In Vitro Study

Feng Zhang, DDS, PhD
- Resident Doctor, Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Gou-li Yang, DDS, PhD
- Attending Doctor, Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Fu-ming He, DDS, PhD
- Associate Chief Physician, Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Li-juan Zhang, DDS
- Resident Doctor, Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Shi-fang Zhao, MD, DDS
- Professor, Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou, China
- Address correspondence and reprint requests to Dr Zhao: Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, 395 Yan'an Road, Hangzhou, China 310006

published online 04 March 2010.

Purpose

The purpose of this study was to investigate the effect of surface chemistry of a sandblasted and acid-etched implant (with and without titanium hydride [TiH₂]) on cell attachment, proliferation, and differentiation of preosteoblasts (MC3T3-E1).

Materials and Methods

Sandblasted and dual acid-etched titanium discs comprised the test group, whereas sandblasted, acid-etched, and heat-treated discs comprised the control group. Both groups' discs were sent for surface characterization. MC3T3-E1 cells were cultured on these 2 groups' discs, and then cell attachment, cell proliferation, and cell differentiation were analyzed.

Results

Scanning electron microscope analysis showed that the titanium discs in the 2 groups shared the same surface topography; however, x-ray diffraction examination showed that the TiH₂ diffractions only appeared in the test group. Cell attachment and cell proliferation were much better in the test group than in the control group at all time points investigated (P < .05). The expressions of alkaline phosphatase and osteocalcin were significantly higher in the test group than in the control group for both protein and transcription level at every time point (P < .05 or P < .01).

Conclusions

These results suggested that surface chemistry played a significant role in cell response to the sandblasted and acid-etched surface and the presence of TiH₂ might promote the attachment, proliferation, and differentiation of preosteoblasts.